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Fig. 1 | BMC Biology

Fig. 1

From: Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells

Fig. 1Fig. 1

A Gating scheme to identify major immune cell types, B ridgeline plots to identify thresholds, C antibody-based UMAP of major cell types, and D age projection (above and below median age, 49 years) onto NK and B cell UMAPs. PBMCs from 32 WIHS participants were hashtagged and stained with 40 oligonucleotide-tagged antibodies. The major immune cell types were UMAP-Louvain-clustered by CD3, CD19, CD14, CD16, CD20, CD56, CD123, and CD206 surface expression. Then, each major known cell type was UMAP-Louvain-clustered separately by all non-negative surface markers. Classical monocytes (CM) formed 7 clusters, intermediate monocytes (INT) 3, and nonclassical monocytes (NCM) 3 clusters. CD4+ T cells formed 13 clusters and CD8+ T cells formed 14. NK cells formed 3 clusters and B cells formed 7 clusters. Numbers of clusters are indicated in each UMAP. Green: above median, red: below median

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BMC Biology

ISSN: 1741-7007

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