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Fig. 3 | BMC Biology

Fig. 3

From: Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and decontamination methods

Fig. 3

Performance of non-WGA-based DNA library preparation methods. a Read composition of raw data. b Library conversion rate. *p < 0.05, **p < 0.01, Wilcoxon tests. c Duplication rate of sequins. d JSD between the expected compositions of sequins and measured profiles including sequins and microbial contaminants (left) or measured sequins profiles (right). e JSD between the measured profiles of experimental replicates. f Evaluation of methods based on three crucial parameters. Fidelity of designated reads was defined as 1 subtracted by JSD between the expected composition of sequins and measured sequins profiles, and reproducibility was defined as 1 subtracted by JSD between the measured profiles of experimental replicates

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BMC Biology

ISSN: 1741-7007

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