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Fig. 4 | BMC Biology

Fig. 4

From: Pheromone sensing in Drosophila requires support cell-expressed Osiris 8

Fig. 4

Functional analysis of Osi8 reveals a selective role in pheromone sensing. A Schematic of the generation of the Osi81 mutant. Osi8 exons and UTRs are shaded dark and light green, respectively. B Osi8 RNA FISH on whole-mount antennae from control (w1118) and Osi81 mutant animals. Scale bar, 20 μm. C Scanning electron micrographs of antennae from control (w1118) and Osi81 mutants (2-day-old animals). Scale bars, 50 μm. The higher-magnification images (scale bars, 10 μm; samples from 20-day-old animals) highlight several trichoid sensilla on the distal edge of the antenna; the basal drums of these sensilla are indicated with yellow arrowheads. D Representative traces of electrophysiological responses of Or47b OSNs to palmitoleic acid (10−1 v/v) (0.5 s stimulus, gray bar) in control (w1118), Osi8 mutant (Osi81), control rescue (UAS-CD4:tdTomato/+;Osi81/Osi81-DsRed,Osi8-Gal4), and Osi8 rescue (UAS-Osi8/+;Osi81/Osi81-DsRed,Osi8-Gal4) animals. Raster plots and peristimulus time histograms (PSTHs) of these responses are shown below each trace. Line width in the PSTH represents the SEM. E Dose-response curves of Or47b OSN responses to palmitoleic acid; genotypes are color-coded as in D. Mean responses ±SEM are plotted. n = 10 sensilla; 4–5 flies. Statistical comparisons between genotypes were performed by two-way ANOVA: NS P > 0.05, *** P < 0.001. Full statistical analyses are provided in Additional file 10: Data S2. F Representative traces, raster plots, and PSTHs of electrophysiological responses of Or88a OSNs to methyl palmitate (10−1 v/v) (0.5 s stimulus, gray bar) in the same genotypes as in D. G Dose-response curves of Or88a OSN responses to methyl palmitate; genotypes are color-coded as in D. Mean responses ±SEM are plotted; n = 10 sensilla; 4–5 flies. Statistical analyses were performed as in E. H Representative traces, raster plots, and PSTHs of electrophysiological responses of Or67d OSNs to cis-vaccenyl acetate (10−1 v/v) (0.5 s stimulus, gray bar) in control (w1118) and Osi8 mutant (Osi81) animals. I Dose-response curves of Or67d OSN responses to cis-vaccenyl acetate; genotypes are color-coded as in H. Mean responses ±SEM are plotted; n = 12 sensilla; 4–6 flies. Statistical analyses were performed as in E. J Basal spiking frequency of the indicated OSNs for control (w1118) and Osi8 mutant (Osi81) animals; n = 12 sensilla, from 3 flies. t-test: NS P > 0.05

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