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Fig. 4 | BMC Biology

Fig. 4

From: Circadian rhythms in the plant host influence rhythmicity of rhizosphere microbiota

Fig. 4

Rhythmicity of bacterial and fungal OTUs in constant conditions. A Experimental design. Plants were grown for 6 weeks under 12L12D cycles before sampling started. Four replicate root and rhizosphere samples were collected every 3 h, each derived from 8 plants. After 24 h, plants were transferred to constant light. Sampling was resumed at time 48 h for another 2 days, in order to test for free-running rhythmicity in constant conditions. B Temporal expression patterns of the LUX mRNA in wild-type and lhy-ox mutant roots. Benjamini–Hochberg p-values (BHQ) are given. C Temporal changes in relative abundance of individual bacterial OTUs (red) and fungal OTUs (blue) in constant light. Rhythmic OTUs were identified based on period values between 18 and 30 h and BHQ < 0.05. Left-hand panels show their rhythmic changes in the rhizosphere of wild-type plants. Right-hand panels show disrupted rhythmicity for the same OTUs in the rhizosphere of lhy-ox plants. Each rectangle represents the relative abundance of one OTU at a particular time point, averaged from 4 replicate samples each composed of 8 plants. White and black rectangles below the chart indicate days and nights, respectively. Grey rectangles indicate subjective nights in constant light conditions. Lists of rhythmic OTUs detected in this experiment are provided as Additional File 4

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