Fig. 4

Increased heterochromatin formation in the fat body promotes imd pathway mediated DptA and Dro overexpression. A–C Relative expression of imd-mediated AMP mRNA in the fat body was measured before PA14 infection. n= 8 flies/group. D–H Relative expression of HP1a, imd, DptA, Dro, and AttA mRNA in the fat body was measured in the early and middle stages of PA14 infection. Note that C564>HP1a flies already showed constitutive upregulation of various AMP as compared to control C564>+ before infection as shown in A–C but the differences are not easily observed in D–H due to the log scale. n=4 flies/group at each time point. Data are shown as mean ± SD and **p < 0.01, ***p < 0.001 by Student’s t-test. ns, not significant