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Fig. 3 | BMC Biology

Fig. 3

From: Astrocytes in CA1 modulate schema establishment in the hippocampal-cortical neuron network

Fig. 3

CA1 astrocyte Gi activation impairs the initial schema memory by inhibiting the activation of CA1-ACC projecting neurons (c-Fos expression). A Schematic of the experimental protocol. B Performance index of Cohort 1 rats during the learning of OPA and NPA in hM4Di_CNO and hM4Di_Saline groups (n=6 each). CNO or saline was intraperitoneally injected during session 1 to session 5. C Nonrewarded cued-recall probe tests (PT1–3) of Cohort 1 rats for the acquisition of OPAs across sessions 6, 15, and 23 in hM4Di_CNO and hM4Di_Saline groups (n=6 each). The graph represents the percentage of dig time at the cued location (light color bars) relative to that of the non-cued locations (dark color bars). D Memory recall in PT4 of Cohort 1 rats for the NPA (light color bar) in hM4Di_CNO and hM4Di_Saline groups (n=6 each). E Schematic of CA1-ACC projecting neurons experiment: AAV2-retro-CaMKII-Cre was injected into the ACC, and AAV2/9-ef1α-DIO-EYFP together with AAV8-GFAP-hM4Di–mCherry were injected into the CA1; scale bar, 20 µm. F EYFP-positive axons of CA1 projection neurons in the ACC; scale bar, 100 µm. G Representative images of hM4Di in astrocytes (red), EYFP in ACC projecting CA1 neurons (green) and c-Fos (magenta) in the CA1 of saline-injected Cohort 2 rats (G) or CNO-injected Cohort 2 rats in session 1 (H); white neurons that arrows point to are overlap of EYFP and c-Fos; scale bar, 20 µm. I Representative images of c-Fos (red) in ACC of saline-injected Cohort 3 rats in session 1 (I) or CNO-injected Cohort 3 rats in session 1 (J); scale bar, 100 µm. K Comparison of c-Fos expression level in CA1 between hM4Di_CNO and hM4Di_Saline groups of Cohort 2 rats in session 1 (n=6 each). L Comparison of the percent of CA1 cells projecting into the ACC that express c-Fos between hM4Di_CNO and hM4Di_Saline groups of Cohort 2 rats in session 1 (n=6 each). M Comparison of c-Fos expression level in ACC between hM4Di_CNO and hM4Di_Saline groups of Cohort 3 rats in session 1 (n=6 each). Data are presented as the mean ± SEM, *p<0.05, **p<0.01

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