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Fig. 2 | BMC Biology

Fig. 2

From: ERF49 mediates brassinosteroid regulation of heat stress tolerance in Arabidopsis thaliana

Fig. 2

The structure and characterization of ERF49. a The gene and protein structure of ERF49. The exon is denoted by a black box. 5′-UTR and 3′-UTR are denoted by white boxes. The translation initiation codons are shown. aa means amino acids and bp means base pair. b GUS staining assay of ERF49pro::gERF49-GUS transgenic plants in different tissues (from left to right: 14-day-old seedling, rosette leaf (upper), 5-day-old seedling (upper), silique (lower), inflorescence (upper), and cauline leaf (lower)). Scale bars, 5 mm. c Analysis of ERF49 expression level in different organs by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). The expression level in the seedlings is set to 1.0, and error bars represent standard deviations of three biological replicates. Numerical data is provided in Additional file 6. d Confocal micrographs of subcellular localization of reporter signal exhibited by 35S::ERF49-GFP in tobacco leaf. Scale bars: 50 μm

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