Fig. 3

ODE model captures the characteristic steps in DSBR. a Sketch of the parameters of our ODE model that describe the whole DNA repair process, as already illustrated in Fig. 1a: “modified” cells (m) are submitted to metabolism change upon DNA repair induction (for a time period \(\tau\)) and DSB (at the rate \(\beta\)) and thus becoming “broken” cells (b); they do DSBR (at the rate \(\rho\)) and express GFP (after a time period \(\gamma\)), thus becoming GFP+ cells (g); and they divide (at the rate \(\alpha\)). b Molecular measurements are performed by Southern blotting at regular time intervals (2 h) on samples from a bulk cell culture. They provide information on the fraction of cells in a population that have done DSB, but not yet repaired, and the fraction of cells that have completed repair. c Prior and posterior parameter distributions of metabolism change (\(\tau\)), DSB rate (\(\beta\)), DSBR rate (\(\rho\)), and cell division rate (\(\alpha\)). d Comparison of ODE model predictions with molecular data for the case NR-Cas9. In plots, the black dots represent the molecular data, while the solid lines represent the simulations from the ODE model