Fig. 4.

ALI1 protein is localized at the cell’s periphery in PM, ER, and tonoplast and does not translocate after oxo-C14-HSL treatment. Confocal images displaying the subcellular localization of YFP-tagged ALI1 before and after oxo-C14-HSL treatment when transiently expressed in cells of N. benthamiana leaves. Plasmids carrying YFP-tagged ALI1 version and mCherry-marked proteins localizing in ER (AtWAK2-HEDL), tonoplast (c-TIP), or plasma membrane (AtPIP2) were co-transformed to N. benthamiana leaf epidermal cells by Agrobacterium-infiltration, and further re-infiltrated after 24 h at the same co-transformed spots of a leaf with 6 µM oxo-C14-HSL. The left panel shows in green, fluorescence of YFP-tagged ALI1, whereas the middle panel shows in red, fluorescence of differently localized mCherry-marked proteins (Additional file 2: Table S3). The right panel shows corresponding merged images, and yellow color indicates colocalization. Scale bar = 40 μm