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Fig. 5 | BMC Biology

Fig. 5

From: De novo emergence, existence, and demise of a protein-coding gene in murids

Fig. 5

D6Ertd527e knock-out analysis. a Schematic depiction of positions of designed CRISPR cleavage points. b A UCSC browser snapshot of data from RNA-seq libraries from oocytes from three wild-type mouse and three mutants. Low residual signal in the coding sequence in D6Ertd527e mutants can be explained by multimapping repetitive reads originating from other loci. c Breeding performance of matings with different combinations of genotypes. p-values were calculated with two-tailed t-tests. d PCA analysis of RNA-seq libraries suggests higher variability of wild-type controls and clustering of mutant transcriptomes. e MA plot depicting differentially expressed genes in D6Ertd527e mutant oocytes. Significantly upregulated and downregulated transcripts are shown in red and blue, respectively. The most abundant significantly changed transcripts were Gm20763 and Ccnd2

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