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Fig. 4 | BMC Biology

Fig. 4

From: Experimental evidence for enzymatic cell wall dissolution in a microbial protoplast feeder (Orciraptor agilis, Viridiraptoridae)

Fig. 4

Distribution of the anti-GH5_5 pAb (pAb), F-actin (PHA) and cellulose (CFW) during different stages of attack by Orciraptor agilis on Actinotaenium cf. silvae-nigrae. A Attached Orciraptor cell with lysopodium, 10 min a.a.a. B Attached Orciraptor cell with lysopodium and pronounced GH5_5 signal at the algal cell wall, 1 h a.a.a. C Attached Orciraptor cell with lysopodium and pronounced GH5_5 signal at the indented algal cell wall (and at some intracellular structures), 2 h a.a.a. Orciraptor cell with pseudopodium extending into the Actinotaenium cell (the vertical line visible in the CFW-stained algal cell is out-of-focus fluorescence of the isthmus). The GH5_5 signal localises to the hole in the algal cell wall (and to some intracellular structures), 4 h a.a.a. Fluorescence micrographs in A, B and D represent single focal planes captured by widefield microscopy, in C captured by confocal laser scanning microscopy. Scale bars = 5 μm

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