Fig. 6

Effect of the anti-GH5_5 pAb on the feeding process of Orciraptor agilis. A Percentage of emptied algal cells in usual cultures (condition 1) and those supplemented with the anti-GH5_5 pAb (condition 2), with the anti-GH5_5 pAb plus the OaGH5_5P enzyme (condition 3), and the boiled anti-GH5_5 pAb (condition 4). B Pectin and cellulose distribution in an Actinotaenium cell from condition 2 visualised by fluorescence staining (JIM5 = pectin; CFW = cellulose). C Pectin and cellulose distribution in an Actinotaenium cell which did not have contact to Orciraptor (JIM5 = pectin; CFW = cellulose). D Actinotaenium cell from condition 2 with at least one attempted attack (dashed rectangle) and two incomplete perforations (arrows), SEM. E Close-up of the attempted perforation shown in D reveals large areas of exposed cellulose microfibrils, SEM. Fluorescence micrographs in B and C represent Z-projections of image data from widefield microscopy. Scale bars in B, C = 5 μm, in D = 2μm, in E = 1 μm