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Fig. 4 | BMC Biology

Fig. 4

From: The genomic basis of copper tolerance in Drosophila is shaped by a complex interplay of regulatory and environmental factors

Fig. 4

Copper survival experiments for all ten copper candidate genes. LT100 values comparing candidate gene disruption and knockdown lines with their genetic background controls (3 to 5 biological replicates of 15 females). Significant LT100 assessed with a two-sided t-test, with p-values < 0.05 are shown with *, p-values < 0.01 with **, and p-values < 0.001 with ***. Del is deletion and GDP is gene disruption. For RNAi knockdowns, genes thought to act in the ‘detox’ tissues—including the gut fat body and Malphigian tubules—were targeted with the 6g1HR-Gal4 driver, while those genes whose expression was more gut specific were targeted with MexG-Gal4. A ubiquitous Actin5C-Gal4 knockdown was used for all other crosses. Driver abbreviations are as follows: MexG-Gal4 w1118 is the introgressed w1118 version of MexG-Gal4 driver, HR is the HikoneR driver, and Act is the Actin5C-Gal4 driver (see Additional file 1: Table S1C)

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