Table 2 Eight out of 10 copper candidate genes play a role in copper tolerance. Fold change (FC) expression after copper treatment in tolerant and sensitive strains. No DE: gene was not differentially expressed. Kaplan-Meier survival curves results and average mortalities. GDP gene disruption line, Del gene deletion line, ⬆ increased mortality, ⬇ decreased mortality, – no data, NS not significant. Crosses with different backgrounds and reciprocal crosses are separated with “;”
Gene | FC in expression after copper treatment | Kaplan-Meier (log-rank test) | LT100 (t-test) | |||||
|---|---|---|---|---|---|---|---|---|
Tol. | Sen. | RNAi | GDP | Del | RNAi | GDP | Del | |
CG11594 | 2.06 | 1.84 | ⬆<0.001, <0.001 | – | ⬆< 0.001 | ⬆0.04, 0.004 | – | ⬆0.002 |
Cyp6w1 | 2.03 | no DE | ⬆<0.001, 0.028 | – | – | ⬆0.032, 0.059 | – | – |
Cyp6a8 | No DE | 1.91 | – | ⬆< 0.001 | – | – | ⬆<0.001 | – |
Sodh-1 | 4.22 | 3.46 | ⬆<0.001 | – | – | ⬆<0.001 | – | – |
CG6910 | 2.86 | 3.09 | NS; NS | NS | – | NS; NS | ⬆0.031 | – |
Jon65Aiv | −1.83 | −4.02 | ⬇0.0062, <0.001; NS | – | – | NS; NS | – | – |
CG32444 | 3.84 | 2.70 | ⬆<0.001 | – | – | – | NS | – |
CG5966 | 4.07 | 4.64 | ⬇<0.001, <0.001 | – | – | NS | – | – |
CG5773 | −2.24 | −5.13 | NS | – | – | NS | – | – |
Cyp4e3 | 3.81 | 2.05 | NS; NS | – | – | NS; NS | – | – |