Table 1 Primer sequences
Gene | Oligo names | Primer sequences 5′-3′ |
|---|---|---|
| Â | CdICE1-B | ACTGGATCCATGCTACCGGAAAACGACA |
CdICE1-S | TCCCCCGGGAATGGCACCATGATAACCT | |
| Â | zh-F | CGGGTACCATGCTACCGGAAAACGACA |
zh-R | TCCCCGCGGTAATGGCACCATGATAACCT | |
| Â | ICE1 | ACTCATATGATGCTACCGGAAAACGACA |
| Â | ICE21 | ACTCATATGACCCCTACAACCACCCCA |
| Â | ICE85 | ACTCATATGTCACCTTCACAATCACACTCAC |
| Â | ICE121 | ACTCATATGTGTGACCCTAGCTTCATTTCCA |
| Â | ICE471 | ACTGGATCCAATGGCACCATGATAACCT |
CdICE1 | CdICE-F | CCTACCCAACAAATGGCAACTA |
CdICE-R | TCTCTCCACTCTGTCTCAGCGC | |
CBF1 | cbf1-F | GTGACGTGTCGCTTTGGAGTTAC |
cbf1-R | GTGAAGCAAAGAAGTAGAAAACG | |
CBF2 | cbf2-F | TCGAGGGAGATGATGACGTGTCC |
cbf2-R | TATTTTGATTTGTTGCTTATGG | |
CBF3 | cbf3-F | CGACGGCGATGATGACGACGT |
cbf3-R | GCATTTAAGAATAGCCCACAC | |
COR6.6 | cor6.6-F | CAGAGACCAACAAGAATGCC |
cor6.6-R | CGATATACTCTTTCCCGCCT | |
COR15a | cor15a-F | AAAGCAGGAGAGGCTAAGGAT |
cor15a-R | CATGAAGAGAGAGGATATGGATCA | |
RD29a | rd29a-F | TAGGAAAGTAAAGGCTAGAGCTAAG |
rd29a-R | AATCGGAAGACACGACAGG | |
β-tubulin | tubin-F | AAGATTCGTCCCACGCG |
tubin-R | TCCTTTAGCCCAATTGTTACC | |
U6 | U6-F | CTCGCTTCGGCAGCACA |
U6-R | AACGCTTCACGAATTTGCGT | |
CSD2 | CSD2-F | AACCCTAACAACATGACACACG |
CSD2-R | GAACCACAAAGGCTCTTCCAAC | |
CSD1 | CSD1-F | AGACCCTGATGACCTCGGAAA |
CSD1-R | GCCACACACCAGAAGATACAC | |
miR398 | miR398-F | TGTGTTCTCAGGTCACCCCT |
Uni-miR qPCR Primer | From TaKaRa | |
MIR398a | miR398a-F | AGAAGAAGAGAAGAACAACAGGAGGTG |
miR398a-R | TTTAGTAAGGTGAAAAAATGGAACAGG | |
MIR398b | miR398b-F | TAACAAGAAGATATCAATATATCATG |
miR398b-R | ACCATTTGGTAAATGAGTAAAAGCCAGCC | |
MIR398c | miR398c-F | TCGAAACTCAAACTGTAACAGTCC |
| Â | miR398c-R | ATTTGGTAAATGAATAGAAGCCACG |