Figure 4
Sessile hemocytes interact with autolysing swimming muscles. (A) Fluorescence overlay showing hemocytes (red, CM-DiI) evenly distributed throughout a portion of the dorsal abdominal wall of a two-day-old naïve mosquito. DNA is stained blue with Hoechst 33342. (B) Fluorescence overlay of a portion of the dorsal abdomen of a two-day-old naïve mosquito showing the heart, the alary muscles, and the remnants of larval swimming muscles undergoing autolysis (white arrows). Muscle is stained green with AlexaFluor-488-conjugated phalloidin and DNA is stained blue with Hoechst 33342. (C) Higher magnification fluorescence overlay of hemocytes (red) engaged in an apparent interaction with the condensed nuclear material (blue; for example, arrows) of autolysed abdominal myocytes (green; for example, yellow arrows) in a two-day-old naïve mosquito.