Figure 2From: Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23Altered expression of Notch signaling target genes in awd clones. (A and C) Stage 7–8 egg chambers were dissected from hs-flp; +/+; Ubi-GFP, FRT82B/FRT82B, awdj2A4 females, and the awd mutant clones were identified by lack of GFP staining (green). (B, D-E) Stage 7 egg chambers were dissected from hs-flp/GbeSu(H) m8 -lacZ; act-Gal4, UAS-GFP/+; FRT82B, act-Gal80/FRT82B, awdj2A4 females. In (A-C) the awd mutant clones were identified by lack of Awd or GFP staining (green), while in (D-E) the awd mutant clones are GFP positive. In all panels, awd mutant clones are marked with dashed lines. (A) Cyclin B (red), (B) Cut (red) and (C) Fasciclin III (FasIII; in red) are normally negatively regulated by Notch signaling, but up-regulated in awd mutant clones (lack of GFP or Awd; arrows). (D) Hindsight (Hnt; in red) is normally induced by Notch signaling, and is down-regulated in awd clones (GFP-positive; empty arrowhead). (E) The lacZ reporter gene expression driven by Notch-activated Su(H) promoter [GbeSu(H) m8 -lacZ] is lost in awd clones (GFP-positive; empty arrowhead). Bars are 20 μm.Back to article page