Figure 6From: Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shhin the diencephalon in vertebratesLoss of Pax6 results in precocious formation and expanded expression of Shh in the MDO. (A-H) ISH for Shh and Ptch1 on coronal sections of E12.5 embryos of the indicated genotypes. (I and J) Wholemount ISH for Shh in control (I) and Pax6Sey/Sey(J) embryos at E9.5. Arrows denote the MDO; brackets show the expanded domain of Shh in the MDO; dashed line arrow indicates the dorsally (rostrally) and ventrally (caudally) expanded Ptch1 expression domains in F; the red arrowhead in J indicates the precocious induction of the ZLI Shh expression in Pax6Sey/Sey embryos. Scale bar in (A): 200 μm (A-H). E, embryonic day; ISH, in situ hybridization; MDO, mid-diencephalic organizer; ZLI, zona limitans intrathalamica.Back to article page