Cross | Parents | Â | Progeny | Â | Â | Â | Â | Â | Â | Â | Â | Â |
---|
 | Male | Female | Tc | Genotype | Egg | L1 | L2 | L3 | L4 | Pupae | Adults | % |
---|
a | LA513A/+ | +/+ | Yes | LA513A/+ | 1000 | 489 | 468 | 446 | 442 | 437 | 434 | 89 |
 |  |  |  | Wild type |  | 444 | 431 | 403 | 400 | 396 | 392 | 88 |
b | +/+ | LA513A/+ | Yes | LA513A/+ | 1000 | 442 | 420 | 404 | 399 | 393 | 383 | 87 |
 |  |  |  | Wild type |  | 466 | 444 | 428 | 417 | 412 | 404 | 87 |
c | LA513A/+ | +/+ | No | LA513A/+ | 540 | 274 | 265 | 235 | 208 | 155 |
7
|
2.6
|
 |  |  |  | Wild type |  | 233 | 225 | 214 | 212 | 209 | 206 | 88 |
d | +/+ | LA513A/+ | No | LA513A/+ | 497 | 216 | 205 | 181 | 168 | 131 |
9
|
4.2
|
 |  |  |  | Wild type |  | 241 | 225 | 216 | 214 | 211 | 207 | 86 |
- Adults heterozygous for LA513A were allowed to mate with wild type. a and c: transgenic males crossed to wild type females; b, d: the reciprocal cross using transgenic females. Eggs were collected and the resulting larvae reared in media supplemented with tetracycline (Tc) to 30 μg/ml (crosses a and b) or in normal, tetracycline-free media (crosses c and d). Data are the sum of at least 5 experiments. The ratio of transgenic to non-transgenic first instar larvae (L1) was approximately 1:1 (1421:1384), indicating that there was no significant differential embryonic mortality between transgenic and wild type (binomial exact test,p = 0.248). The numbers of transgenic and non-transgenic first, second, third and fourth instar larvae (L1–L4), pupae and adults resulting from these eggs is shown. In the absence of tetracycline, the transgenics showed very high (96–97%) mortality between first instar larvae and adult stages (highlighted cells); this was completely suppressed by tetracycline. Mortality of transgenics in the absence of tetracycline is strongly stage-specific, being primarily around the pupal stage (L4-pupae and pupae-adult; most affected individuals started to pupate but failed to develop beyond the earliest stages of pupal development).