From: The diversity of reproductive parasites among arthropods: Wolbachiado not walk alone
Organism | Gene | Primer (5' – 3') | Annealing temperature | Positive control | Size | Reference |
---|---|---|---|---|---|---|
Arthropods | 18S rDNA | NSF4/18 – CTGGTTGATYCTGCCAGT * | 54°C | Any arthropod | 400–450 bp | |
 |  | NSR399/19 – TCTCAGGCTCCYTCTCCGG * |  |  |  |  |
Rickettsia sp. | 17-kDa | R1 – GCTCTTGCAACTTCTATGTT * | 54°C | Adalia decempunctata | 434 bp | [67] |
 |  | R2 – CATTGTTCGTCAGGTTGGCG * |  |  |  |  |
Wolbachia pipientis | 16S rDNA | 16Swolb76-99f – TTGTAGCCTGCTATGGTATAACT * | 54°C | Culex pipiens | 896 bp | [74] |
 |  | 16Swolb1012-994r – GAATAGGTATGATTTTCATGA * |  |  |  |  |
 | wsp | 81F – TGGTCCAATAAGTGATGAAGAAAC | 50°C | 81F/522R: Aedes albopictus – wAlbB | 442 bp | [75] |
 |  | 136F – TGAAATTTTACCTCTTTTC |  | 172F/691R: Aedes albopictus – wAlbA | 514 bp |  |
 |  | 172F – ACCTATAAGAAAGACAAG |  | 81F/484R: Protocalliphora sp. – wA2 | 380 bp |  |
 |  | 484R – TTTGATCATTCACAGCGT |  | 136F/691R: Protocalliphora sp. – wA1 | 516 bp |  |
 |  | 522R – ACCAGCTTTTGCTTGATA |  |  |  |  |
 |  | 691R – AAAAATTAAACGCTACTCCA |  |  |  |  |
Arsenophonus nasoniae | 16S rDNA | ArsF – GGGTTGTAAAGTACTTTCAGTCGT * | 52°C | ArsF, ArsF3/ArsR2, ArsR3: Nasonia vitripennis | 581–804 bp | This study |
 |  | ArsF2 – CCCTAAGCTTAACTTAGGA * |  | ArsF2/ArsR2: Polistes nympha | 612 bp |  |
 |  | ArsF3 – GTCGTGAGGAARGTGTTARGGTT * |  |  |  |  |
 |  | ArsR2 – GTAGCCCTRCTCGTAAGGGCC * |  |  |  |  |
 |  | ArsR3 – CCTYTATCTCTAAAGGMTTCGCTGGATG * |  |  |  |  |
Cardinium hertigii | 16S rDNA | CLO-f1 – GGAACCTTACCTGGGCTAGAATGTATT * | 54°C | Holocnemus pluchei | CLO-f1/CLO-r1: 466 bp | [13] |
 |  | CLO-r1 – GCCACTGTCTTCAAGCTCTACCAAC * |  |  | ChF/CLO-r1: 953 bp |  |
 |  | ChF – TACTGTAAGAATAAGCACCGGC |  |  |  | [12] |
Flavobacterium sp. | 16S rDNA | FlavF – CGAATAAGTRTCGGCAAACTCCG * | 52°C | Coleomegilla maculata | 530 bp | This study |
 |  | FlavR – CTAAACTRTTTCYAGCTTATTCG * |  |  |  |  |
Spiroplasma ixodetis | 16S rDNA | SpixoF – TTAGGGGCTCAACCCCTAACC * | 52°C | Adalia bipunctata | 810 bp | This study |
 |  | SpixoR – TCTGGCATTGCCAACTCTC * |  |  |  |  |
S. poulsonii | 16S rDNA | SpoulF – GCTTAACTCCAGTTCGCC * | 55°C | Drosophila melanogaster | 421 bp | [42] |
 |  | SpoulR – CCTGTCTCAATGTTAACCTC * |  |  |  |  |