Figure 5
From: Effect of hydroxyurea on the promoter occupancy profiles of tumor suppressor p53 and p73
Integration of chromatin immunoprecipitation on DNA chip and gene expression profiling. (a) Small interfering RNA (siRNA)-mediated knockdown of p53 and/or p73 in HCT16-3(6) cells. Immunoblot of lysates from untreated or hydroxyurea-treated HCT116-3(6) cells (1 mM, 16 hours) following transfection with chemically synthesized siRNA oligos against lacZ, p53, and/or p73. (b) Flow chart for the integrated analysis of chromatin immunoprecipitation on DNA chip with expression profiling. (c) Summary of the relationship between p53/p73 binding and gene expression. Column 4: expression in p53 and p73 siRNA-transfected cells is lower than that of lacZ-transfected cells. Column 5: expression in p53 and p73 siRNA-transfected cells is higher than that of lacZ-transfected cells.