Figure 4

Chromatin immunoprecipitation (ChIP) assays of the peroxisome proliferators activated receptor γ (PPARγ) promoter region during adipogenesis. 3T3-L1 cells were harvested at the indicated times, and 106 cells were used as the input for each assay. DNA fragments immunoprecipitated by the indicated antibody were recovered and amplified by the primers designed for the PPARγ promoter region (see Figure 1). A total of 1% of the input was also amplified without ChIP, and is shown at the bottom.