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Figure 1 | BMC Biology

Figure 1

From: A Dictyostelium chalone uses G proteins to regulate proliferation

Figure 1

The effect of recombinant AprA (rAprA) on cell proliferation. Cells were grown in the presence or absence of rAprA for 12 h, and the percent decrease in cell density caused by rAprA was calculated. When compared with the inhibition of wild-type (WT) cell proliferation, the inhibition of gα1 - , gα2 -, gα3 -, gα5 -, and gα7 - cells is not significant with P > 0.05 (one-way ANOVA, Dunnett's test), whereas the inhibition of gα4 - is significant with P < 0.01, and the inhibition of gα8 -, gα9 -, and - cells is significant with P < 0.001. The inhibition values for gα8 -, gα9 -, and - cells are not significantly different between each other with P > 0.05 (one-way ANOVA, Tukey's test), but the inhibition of Gα4 - cells is significantly different from that of gα8 -, gα9 -, and - cells with P < 0.01 (one-way ANOVA, Dunnett's test). Values are the mean ± s.e.m. from at least four separate experiments.

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