A Dictyostelium chalone uses G proteins to regulate proliferation

Table 2 The effect of G proteins on the mass and protein content of cells

	Per 10⁷ cells		Percent of cells with n nuclei			Per 10⁷ nuclei
Cell type	Mass, mg	Protein, mg	1	2	3 +	Nuclei/100 cells	Mass, mg	Protein, mg
Wild-type	10.8 ± 0.6	0.32 ± 0.01	76 ± 3	21 ± 2	3 ± 1	129 ± 5	8.4 ± 0.6	0.25 ± 0.01
gα2 ^-	10.7 ± 0.3	0.33 ± 0.01	72 ± 3	22 ± 2	6 ± 2	134 ± 6	8.0 ± 0.5	0.25 ± 0.02
gα5 ^-	12.1 ± 0.5	0.25 ± 0.01	59 ± 6	31 ± 4	11 ± 3	154 ± 8	7.9 ± 0.6	0.16 ± 0.01*
gα7 ^-	8.8 ± 0.3	0.28 ± 0.01	68 ± 6	25 ± 4	6 ± 2	140 ± 9	6.3 ± 0.5	0.20 ± 0.01
gα8 ^-	15.5 ± 0.1**	0.40 ± 0.02	56 ± 5*	30 ± 4	14 ± 2**	166 ± 8*	9.3 ± 0.5	0.24 ± 0.02
gα9 ^-	16.3 ± 1.5**	0.42 ± 0.01*	78 ± 4	20 ± 3	2 ± 1	124 ± 4	13.2 ± 1.3**	0.34 ± 0.02*
gβ ^-	15.8 ± 1.3**	0.47 ± 0.05**	52 ± 5*	32 ± 2	17 ± 2**	180 ± 9**	8.8 ± 0.9	0.26 ± 0.03

The mass and protein content of cells was measured as described in the Materials and Methods, and the percent of cells with 1, 2, 3, or more nuclei was measured by counts of DAPI-stained cells. After calculating the average number of nuclei per 10⁷ cells, the mass and protein per 10⁷ nuclei was calculated. All values are means ± SEM's from three or more independent assays. A * after a value indicates that the value was significantly different from the wild-type value with p < 0.05; ** indicates p < 0.01(1-way ANOVA, Dunnett's test).

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