A high-throughput chemically induced inflammation assay in zebrafish

Table 1 Detection of anti-inflammatory activity using the ChIN assaya

Compound	Mode of action	Concentration (μM)	Significant effect	Reference
Ibuprofen	COX inhibitor	1	***	[38]
		10	***
		50	*
Diclofenac	COX inhibitor	1.5	***	[38, 39]
		3	***
SP600125	JNK inhibitor	20	*	[40]
		50	**
		100	**
		200	***
Trans-resveratrol	COX-1 inhibitor	1	-	[41]
		10	**
		100	***
Mifepristone (RU486)	Progesterone and GR antagonist	1	-	[42]
		10	**
		100	***
Dexamethasone	Steroidal nitric oxide synthase inhibitor	10	-	[43]
		100	**
		1,000	***
Indomethacin	COX inhibitor	1	**	[39]
		10	***
		100	***
Rosiglitazone	PPAR-γ agonist	0.5	*	[44]
		1	**
		10	*
Aspirin	COX inhibitor	10	-	[39]
		20	**
Hydrocortisone	Steroidal GR agonist	1	-	[45]
		10	-
		100	-
		300	*
Sulindac	NS COX-1 inhibitor	1	**	[46]
		10	***
		50	***
		100	***

^aSelected drugs were added to the incubation medium 1 hour prior to addition of copper and were tested at the indicated concentrations for inhibition of leukocyte migration using BACmpx::GFP larvae in chemically induced inflammation assays (ChIn) (10 μM CuSO₄ for 40 minutes). Up to four concentrations were chosen to provide an overview of drug activity in the ChIn assay. We aimed at identifying a concentration yielding significant results with P < 0.001. In some instances, this was not possible (rosiglitazone, aspirin, hydrocortisone) as higher concentrations were lethal or showed reduced significance compared to lower concentrations. All drugs were used in medium containing 1% dimethyl sulfoxide, as were control fish. Asterisks indicate significant leukocyte migration inhibition. ***P < 0.001. **0.001 <P < 0.01. *0.01 <P < 0.05. Minus sign indicates no significant difference. GR, glucocorticoid receptor; NS, nonsteroidal; COX, cyclooxygenase; JNK, c-Jun N-terminal kinase; PPAR-γ, peroxisome proliferator-activated receptor-γ.

ISSN: 1741-7007