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Table 2 A summary of the antibodies selected in this study.

From: A rapid and scalable method for selecting recombinant mouse monoclonal antibodies

 

Target

Antigen

Antibody name

Clone

VL

VH

Formalin sensitive?

Fusion 1

1.1

Jamc.1

 

1.1.1

V14-111+J2

ND

ND

 

1.2

Unc5b

SI1-Unc5b

1.2.1

V17-127+J4

V2-2+D1+J4

Y

   

SI2-Unc5b

1.2.2

V5-48+J5

V1-14+D1+J4

N

    

1.2.3

Cloning halted

 

1.3

Lrrc4a

 

1.3.1

Clone lost

 

1.4

Flrt3

SI3-Flrt3

1.4.1

V3-7+J1

V1-66+D2+J3

N

    

1.4.2

Cloning halted

 

1.5

Appa

 

No positive clone

Fusion 2

2.1

Jamb.1

 

No positive clone

 

2.2

Jamc.1

 

No positive clone

 

2.3

Jamc.2

SI4-Jamc.2

2.3.1

V10-96+J1

V3-2+D2+J1

N

 

2.4

Vasn

 

2.4.1

N.D.

V5-6+D2+J2

ND

 

2.5

Lrrn1

SI5-Lrrn1

2.5.1

V4-57+J5

V1-77+D3+J2

N

  1. The pooled antigens used in the immunizations are numbered, and details of the V, D and J segments identified are listed for each antibody. Antibodies were numerically named with the SI prefix for Sanger Institute.
  2. ND = not determined