Figure 7

tej and tap likely function together for production of cluster-derived and ping-pong piRNAs. (A) Bar plots showing reduction separately for piRNAs mapping to transposons families expressed predominantly in germline, both germline and soma, and predominantly in soma. The tej^48-5-tap¹²⁵ mutants showed a more severe reduction in piRNAs targeting transposons expressed in germline and both germline and soma compared to that in both single mutants. (B) Heat maps representing the ratio of ping-pong piRNAs (10-nucleotide overlap) to piRNA pairs with overlaps of 2 to 26 nucleotides mapping to transposable elements (TEs) of three different subsets: those predominantly expressed in the germline (top panel), in both the germline and soma (middle panel), and predominantly in somatic cells (bottom panel). tap¹²⁵ mutants had a less severe reduction in the ping-pong piRNAs mapping to TEs predominantly repressed in germline than the tej^48-5-tap¹²⁵ mutants, which suffered the most severe loss in ping-pong ratios compared to both single mutants.