Figure 3

Cellular morphology within developing Haloferax volcanii H1206(pJAM1020) static liquid biofilms. (A) Planktonic H. volcanii H1206(pJAM1020) cells from exponential phase shaking Hv-YPC culture. (B,C,D) Cells within a developing H1206(pJAM1020) SL-biofilm grown in a chamber slide (in Hv-YPC medium) after 12 h (B), 2 days (C) and 5 days (D). (E) Table listing the number of cells binning into 2.5-μm categories and summarized statistics for 2,000 cells measured for planktonic and biofilm cell populations (left) and histogram showing distribution of length at 0.2-μm binning intervals for planktonic (black) and biofilm (green) cells (right). Cell length was measured with Fiji particle analysis using images collected from three independent exponential phase shaking cultures and three 12 h biofilms grown in chamber slides. Mean length of populations was statistically different with P < 0.0001. Scale bars equal 20 μm. SD, standard deviation; SL-biofilm, static liquid biofilm.