Figure 4

Time-lapse macroscopic photography of static liquid biofilm reformation. An established 7-day SL-biofilm grown in a plastic Petri dish in rich medium (Hv-YPC) was mechanically homogenized and left to incubate at 42°C while photographs were taken at regular time intervals. (A) Biofilm reformation over a 2-day period, with images shown at 3 h intervals. Inlay: Cellular vitality controls shown as still images from time-lapse series (see Additional files 6 and 7: Movies 5 and 6) of a SL-biofilm treated with heat (left) and with 4% formaldehyde (right). (B) SL-biofilm imaged at 10-min intervals over a 50-min period (a series from between 45 h and 48 h above). Scale bar equals 1 cm. SL-biofilm, static liquid biofilm.