Figure 5

Relief of CsrA repression prevents colicin build-up through increased lysis. Expression histograms of E2crimson through the growth phase of producer cells carrying the mutant pColE2-TT plasmid grown in LB (A), M9-amino acids (B), M9-glucose (C), and M9-glucose + amino acids (D), measured using flow cytometry. Histogram colors correspond to time points along the corresponding growth curve (above), grey lines represent histograms of producers carrying the wild-type plasmid (data from Figure 3A-D). Expression profiles of producers harboring the mutant plasmid have shifted to lower expression values and show a decreased fraction of induced cells when compared to expression distributions of producers carrying the wild-type plasmid.