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Figure 5 | BMC Biology

Figure 5

From: Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression

Figure 5

Eomesa positively regulates mesendoderm and negatively regulates ectoderm markers and chromatin assembly genes. (A) Heatmap of expression differences between wild-type and MZeomesa mutants at sphere stage; π – endoderm; Σ - mesoderm; ∂ - ectoderm. (B) Comparison of all genes, or those up- or down-regulated in MZeomesa embryos compared to wild type with genes that have Smad2 only, common, uncommon or Eomesa only binding within ±10 kb of their TSSs (colour coded as in Figure 3C). Compared to all genes, those that are up- or down-regulated in MZeomesa embryos are significantly associated with lone Eomesa binding. Genes that have common and uncommon binding are associated with down-regulated genes, whilst Smad2 only and common binding is associated with up-regulated genes in MZeomesa embryos. † P ≤2 × 10-5; †† P ≤1 × 10-7; ††† P ≤1 × 10-18. (C) Functional annotation analysis of genes upregulated in MZeomesa mutants. (D) Anatomical analysis of genes downregulated in MZeomesa mutants. (E) Anatomical analysis of genes upregulated in MZeomesa mutants. TSSs, transcription start sites.

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BMC Biology

ISSN: 1741-7007

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