Figure 1
Consensus tree for the DEG/ENaC family. The phylogenetic tree was generated by Bayesian analysis with the program MrBayes 3.2 (see Methods). The numbers at nodes indicate the posterior probabilities computed by MrBayes for the respective node. Scale bar indicates amino acid exchanges per site. Colors indicate the following phyla: blue, Cnidaria; red, Chordata; green, Arthropoda; brown, Nematoda; pink, Mollusca. Abbrevations of species names are as follows: c, chicken (Gallus gallus); h, human (Homo sapiens); Ha, Helix aspersa; Ht, Helisoma trivolvis; l, lamprey (Lampetra fluviatilis), Ls, Lymnaea stagnalis; m, mouse (Mus musculus); r, rat (Rattus norvegicus). Other proteins are from C. elegans, Drosophila melanogaster or Hydra magnipapillata. Accession numbers are as follows: acid-sensitive degenerin (ACD)-1 [GenBank:NM_058894], ACD-2 [GenBank:NM_058892], ACD-4 [GenBank:NM_072829], ACD-5 [GenBank:NM_058795], cASIC1 [EMBL:AY956393], cASIC2 [GenBank:NM_001040467], hASIC1a [EMBL:U78180], hASIC2a [EMBL:U50352], hASIC2b [GenBank:NM_18337], hASIC3 [EMBL:AF095897], hASIC4 [EMBL:AJ271643], lASIC1 [EMBL:AAY28983], rASIC1a [EMBL:U94403], rASIC1b [EMBL:AJ309926], rASIC2a [EMBL:U53211], rASIC2b [EMBL:AB049451], rASIC3 [EMBL:AF013598], rASIC4 [EMBL:AJ271642], hBASIC [EMBL:AJ252011], mBASIC [EMBL:Y19035], rBASIC [EMBL:Y19034], DEG-1 NM_076910; degenerin-like (DEL)-1 [EMBL:U76403], DEL-4 [GenBank:NM_059829], DEL-7 [GenBank:NM_068875], DEL-9 [GenBank:NM_076221], DEL-10 [GenBank:NM-062901], αhENaC [EMBL:X76180], βhENaC [EMBL:X87159], γhENaC [EMBL:X87160], δhENaC [EMBL:U38254], αrENaC [EMBL:X70521], βrENaC [EMBL:X77932], γrENaC [EMBL:X77933], HtFaNaC [EMBL:AF254118], LsFaNaC [EMBL:AF335548], HaFaNaC [EMBL:X92113], fluoride-resistant (FLR)-1 [EMBL:AB012617], HyNaC2 [EMBL:AM393878], HyNaC3 [EMBL:AM393880], HyNaC4 [EMBL:AM393881], HyNaC5 [EMBL:FN257513], HyNaC6 [EMBL:HG422725], HyNaC7 [EMBL:HG422726], HyNaC8 [EMBL:HG422727], HyNaC9 [EMBL:HG422728], HyNaC10 [EMBL:HG422729], HyNaC11 [EMBL:HG422730], HyNaC12 [EMBL:HG422731], mechanosensitive (MEC)-4 [EMBL:X58982], MEC-10 [EMBL:L25312], pickpocket (PPK) [EMBL:Y16225], PPK4 [GenBank:NM_206137], PPK6 [GenBank:NM_137617], PPK7 [GenBank:NM_135172], PPK10 [GenBank:NM_001038805], PPK11 [GenBank:NM_001038798], PPK12 [GenBank:NM_137828], PPK13 [GenBank:NM_001014495], PPK16 [GenBank:NM_001038797], PPK17 [GenBank:NM_135965], PPK19 [EMBL:AY226547], PPK20 [GenBank:NM_143448], PPK21 [GenBank:NM_143447], PPK23 [GenBank:NM_001014749], PPK24 [GenBank:NM_143603], PPK25 [GenBank:NM_206044], PPK26 [GenBank:NM_139868], PPK27 [GenBank:NM_139569], PPK28 [GenBank:NM_001014748], ripped pocket (RPK) [EMBL:Y12640], uncoordinated (UNC)-8 [EMBL:U76402], UNC-105 [GenBank:NM_063301].