Table 2 Microbial strains used in the work
Strain | Genotype | Use | Reference/source |
|---|---|---|---|
H. volcanii H53 | pyrE2Δ trpAΔ | Genomic DNA as target in in vitro mutagenesis | [35] |
H. volcanii H295 | pyrE2Δ bgaHa-Kp trpAΔ mre11Δ rad50Δ | Parental strain for library construction | [37] |
E. coli DH10B | F−endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 80lacZΔM15 araD139 Δ(ara-leu)7697 mcrA (mrr-hsdRM-mrcBC) | Construction of plasmid library | [38] |
E. coli MC1061 | F–araD139 Δ(ara-leu)7696 galE15 galK16 ΔlacX74 rpsL (Strr) hsdR2 (r k −m k +)mcrA mcrB1 | Standard cloning host | [39] |
E. coli DH5α | F−endA1 supE44 thi-1 recA1 relA1 gyrA96 deoR nupG 80lacZΔM15 Δ(lacZYA-argF)U169 hsdR17 (r k −m k +) | Isolation of methylated (Dam+) DNA | Invitrogen |
E. coli GM2929 | F–ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 glnV44 hisG4 rpsL136 (Strr) xyl-5 mtl-1 dam13::Tn9 (Camr) dcm-6 mcrB1 hsdR2 (r k −m k +) mcrA recF143 | Isolation of unmethylated (Dam-) DNA | New England Biolabs |