Figure 8

The Erk-DUSP6-miR181a axis contributes to hyporesponsiveness of NCD4lo T cells. A. Histogram overlays of baseline ex vivo p-Erk (plotted on log on left and linear scale on right) in NCD4lo and NCD4hi cells. B. Pooled data of paired samples showing baseline p-Erk MFI values (Mean ± SE) in NCD4lo and NCD4hi cells. C. ΔΔCt values of Real time RT-PCR for miR-181a normalized to miR-142 on NCD4hi and NCD4lo cells (Mean ± SE from four sets of independently sorted cells). D. 3H-Thymidine incorporation of NCD4lo and NCD4hi cells treated or not with BCI prior to activation (Mean ± SE of triplicate cultures). Data representative of five mice, one of three experiments. E. Relative IFNγ/IL-4 and IFNγ/IL-13 ratios for BCI treated or untreated NCD4lo cells during recall response. Data from three independent experiments shown normalized to respective ratios for NCD4hi cells as 100%, shown as a dotted line. BCI, (E)-2-benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one; DUSP6, dual-specific phosphatase 6; MFI, mean fluorescence intensity; NCD4, naïve CD4 T cells; SE, standard error.