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Fig. 5 | BMC Biology

Fig. 5

From: An La-related protein controls cell cycle arrest by nuclear retrograde transport of tRNAs during diapause formation in Artemia

Fig. 5

Ar-Larp overexpression results in cell cycle arrest in HeLa and MKN45 cells. Transient transfection of Ar-Larp in (a) HeLa and (b) MKN45 cells. Overexpressed green fluorescent protein (GFP) alone, green in control cells; overexpressed GFP-Ar-Larp, green in Ar-Larp-transfected cells. Mitosis was evaluated using the BrdU incorporation assay, the PKH26 label-retaining assay, and immunohistochemistry of Ki67. Cells overexpressing Ar-Larp (white arrows) lacked mitotic activity and remained in cell cycle arrest. c Flow cytometry analysis of the DNA content of HeLa cells using GFP (control) and GFP-Ar-Larp gene transfection. The bar graph shows the transition ratios from G1/S phase to G2/M phase in HeLa cells at 18 and 24 h after GFP-Ar-Larp gene transfection

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BMC Biology

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