Fig. 4

The Z-cad sensor identifies differential plasticity in HMLER EMT subpopulations upon TGFβ1 exposure. a HMLER cells cultured for 24 days with TGFβ1 were sorted into EMT and Bulk^–EMT groups and allowed to grow in the absence of TGFβ1 for 13 days. b Parental HMLER cells were grown sorted into EMT and Bulk^–EMT groups and allowed to grow for 13 days. c HMLER EMT cells were grown in the presence of the indicated concentrations of decitabine (or vehicle) for 5 days. Medium was changed daily with fresh decitabine. Flow cytometry was performed and the EMT fluorescence signature is indicated (n = 3 biological replicates for vehicle and 500 nM; n = 2 biological replicates for 100 nM). d qPCR analysis for the indicated genes at different decitabine concentrations is shown. Vehicle treated values were set to 1.0 for each gene. Each concentration was compared to vehicle and analyzed using an unpaired Student’s t test (n = 3 biological replicates per group). * p value < 0.05, ** p value < 0.01. All scale bars = 20 μm