Fig. 4

dAtg1 is required cell autonomously for Wg expression, but not JNK activation, in undead clones. Late third instar wing discs with mosaic clones positively marked by GFP, anterior is to the left. MMP1 labeling (red in a, c, e and grey in a’, c’, e’) is used as marker of JNK activity. Wg (red in b, d, f and grey in b’, d’, f’) is highly expressed at the dorsal/ventral (D/V) boundaries (arrowheads in b, d, f) of wing discs. (a–b’) Simultaneous expression of hid and p35 in clones. MMP1 expression (arrows in a, a’) is induced in all hid and p35 co-expressing clones. Ectopic expression of Wg (arrows in b, b’) was observed in over 80 % of clones (n = 66) generated in close proximity to the D/V boundaries in the wing discs. Genotype: hs-FLP tub-GAL4 UAS-GFP/UAS-hid; UAS-p35/+; tub-GAL80 FRT80B/FRT80B. (c–d’) Simultaneous expression of hid and p35 in dronc mutant clones. Both MMP1 labeling and ectopic Wg expression, induced by co-expression of hid and p35, are completed blocked in dronc mutant clones (n > 30). Genotype: hs-FLP tub-GAL4 UAS-GFP/UAS-hid; UAS-p35/+; tub-GAL80 FRT80B/droncI29 FRT80B. (e–f’) Simultaneous expression of hid and p35 in dAtg1 mutant clones. hid and p35-induced MMP1 expression persists in dAtg1 mutant clones (arrows in e, e’). In contrast, the ectopic Wg expression induced by hid and p35 is suppressed in over 70 % of dAtg1 clones (n = 73) generated in close proximity to the D/V boundaries in the wing discs. Genotype: hs-FLP tub-GAL4 UAS-GFP/UAS-hid; UAS-p35/+; tub-GAL80 FRT80B/dAtg1 ^Δ3D FRT80B