Fig. 5
From: IMPACT is a GCN2 inhibitor that limits lifespan in Caenorhabditis elegans
Impt-1 knockdown interacts in an age-dependent manner with multiple components of the DR pathway to control longevity. a Lifespan assays of N2 worms, skn-1(zu135), and daf-16(m26) mutants treated with L4440 (control) or impt-1 RNAi from L1. b Lifespan assays of N2 worms and impt-1 +/– (ok3233) mutants treated with L4440 or daf-16 RNAi from day 0 of adulthood. c, d Lifespan assays of N2 worms and impt-1 +/– (ok3233) exposed to L4440 (control) RNAi or skn-1 RNAi from day 0 of adulthood (c) or during larval stages (L1–L4) (d). Values of median lifespan and statistics are reported in Additional file 10: Table S1. Survival curves were compared using the log-rank test. All experiments were repeated at least three times. Data demonstrate the composite of experiments XI–XIII (a), XIV–XVII (b), XVIII–XIX (c) or XX–XXII (d)