Fig. 2

Whitefly genes associated with virus acquisition and transmission. a Heatmap of differentially expressed cathepsin genes (cts) in whiteflies upon acquisition of TYLCV or ToCV after 24, 48 and 72 h, respectively. Three biological replicates were performed for each sample. Color indicates fold change of gene expression (viruliferous/non-viruliferous whiteflies). b Phylogenetic tree of cysteine proteinase-type cathepsins in B. tabaci and other species. Maximum likelihood tree was constructed using amino acid sequences of the peptidase C1A domain. HUMAN cathepsins from human,_MOUSE cathepsins from mouse,_RAT cathepsins from rat,_DROME cathepsins from fruit fly. c Gene clusters containing whitefly-specific genes that were differentially expressed upon ToCV acquisition. Genes marked by asterisk are differentially expressed. Genes in same colors in each cluster are duplicated genes, while genes in white are non-duplicated