Fig. 1

Visualization of clonal neurons using chimeric mice. a Production of chimeric mice from wild-type blastocysts and green fluorescent protein (GFP)-expressing induced pluripotent stem (iPS) cells. Scale bar: 10 mm. b Two examples of the cerebral vesicle in E10.5 chimeric mice observed by bright field (left) and fluorescence microscopy (middle and right). Right panels are magnified views of the boxed area in the middle panels. Arrowheads: GFP-labeled, iPS cell-derived stem cells. Scale bar: 1 mm (left), 500 μm (middle), 50 μm (right). c Distribution of GFP-positive cells in the neocortex of a postnatal day 10 (P10) wild-type chimeric mouse. Scale bar: 1 mm. d iPS-cell-generated GFP-positive cells in the barrel cortex of a P10 wild-type chimeric mouse generated by implanting 10 iPS cells (left) and one iPS cell (right). The neurons (cells with thin processes) and glial cells (cells with bushy processes) derived from iPS cells were visualized by GFP. Scale bar: 100 μm. e Proportion of GFP-positive neurons in the analyzed area in each column after the implantation of 10 iPS cells and one iPS cell. The number of tested columns is shown above the plots. A bar indicates mean value. No significant difference between these two groups was observed; P = 0.73 (t test)