Primers to create DARC-eGFP fusion protein | ||
---|---|---|
 DARC Fwd | 5′- GCC ACC ATG GGG AAC TGT CT | |
 DARC Rvs | 5′-GGA CTT GCC TGC AAG GGC AT | |
 BamH1-DARC Fwd | 5′- GGA TCC GCC ACC GCC ACC ATG GGG AAC TGT CTG TAT C | |
 DARC-linker1 Rvs | 5′- CCC TTG CTC ACC ATC TCG AGG GAC TTG CCT GCA AGG GCA TC | |
 DARC-linker3 Rvs | 5′- CGC CGC GCT GCC GCC GCC GCC GGA CTT GCC TGC AAG GGC ATC | |
 GFP-linker1 Fwd | 5′- TTG CAG GCA AGT CCC TCG AGA TGG TGA GCA AGG GCG AGG AG | |
 GFP-linker3 Fwd | 5′- GGC GGC GGC AGC GCG GCG GCG ATG GTG AGC AAG GGC GAG GAG | |
 GFP-ECORI Rvs | 5′- GAA TTC TTA CTT GTA CAG CTC GTC CAT GCC G | |
Primers for RT-qPCR | ||
 GAPDH Fwd | CCAATGTGTCCGTCGTGGATCT | |
 GAPDH Rvs | GTTGAAGTCGCAGGAGACAACC | |
 DARC Fwd | TCCGGTGGAAAACCTTTCACTA | |
 DARC Rvs | GCTGGTGTCAGGCTGTAGTC | |
 Selp Fwd | TGTTTGGCTTCTGGGATCTGGACA | From Kokkinaki et al. [69] |
 Selp Rvs | AGGCAGCAATTGGGTGCATACAG | |
 Madcam1 Fwd | GACCCATAGAAAGGAGATTCCAGTA | |
 Madcam1 Rvs | TGAGCCCAGTGGAGACTG | |
 Sele Fwd | TGAACTGAAGGGATCAAGAAGACT | From McEver et al. [70] |
 Sele Rvs | GCCGAGGGACATCATCACAT | |
 Chst4 Fwd | TGCCCCACCTCCAAACAT | From Ruddle et al. [71] |
 Chst4 Rvs | GACCAACGCCACGCCTGAGA | |
 Fut7 Fwd | GGACCTCCTCGGGCCACCTACG | From Ruddle et al. [71] |
 Fut7 Rvs | CGCCAAGCAAAGAAGCCACGATAA |