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Fig. 7 | BMC Biology

Fig. 7

From: An RNAi screen of Rho signalling networks identifies RhoH as a regulator of Rac1 in prostate cancer cell migration

Fig. 7

GFP-RhoH rescues the morphological phenotype of RhoH-depleted cells. a Representative images of PC3 cells transfected with GFP, GFP-RhoH-wt or GFP-RhoH-R1 (resistant to siRhoH-1) 48 h after siRNA transfection with siRhoH-1 or control siRNA. Cells were fixed 24 h after plasmid transfection and stained for F-actin with Alexa Fluor 546 phalloidin and for DNA with DAPI. Scale bars 20 μm. b Left, cell area was measured using ImageJ from F-actin-stained images. Scatter dot plots show mean +/− SEM; at least 84 cells/condition were analysed from three independent experiments, n.s. not significant, ***p ≤ 0.001, by one-way analysis of variance (ANOVA). Right, representative immunoblot from cell lysates, probed with the indicated antibodies. GAPDH was used as a loading control

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BMC Biology

ISSN: 1741-7007

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