Fig. 6
From: An adaptable chromosome preparation methodology for use in invertebrate research organisms
Chromosome spreads for different embryonic stages of P. canaliculata. The duration of colchicine treatment on embryos resulted in different densities of mitotic chromosomes for different embryonic stages. a, d, g, j P. canaliculata embryos 5, 6, 8, and 10 dpf, respectively, after removal of egg shell and perivitelline fluid. b, e, h, k Chromosome spreads obtained from embryos at indicated developmental stages incubated in colchicine for 6 h. c, f, i, l Chromosome spreads obtained from embryos at indicated developmental stages incubated in colchicine for 24 h