Fig. 7
From: An adaptable chromosome preparation methodology for use in invertebrate research organisms
Chromosome preparation in the embryos of the sea anemone N. vectensis. The optimized chromosome preparation protocol was used to generate karyotypes for embryos of the sea anemone N. vectensis. a Juvenile stage of N. vectensis. b, c A 128-cell embryo (b) before and (c) after 5 min DI H2O incubation. d Karyotype obtained from N. vectensis embryos after a 30-min incubation in 0.04% colchicine. Notice the incomplete condensation of chromosomes. e Karyotype obtained from N. vectensis embryos after a 45-min incubation in 0.04% colchicine. (2n = 30). f, g Excessive DI H2O incubation or physical force during preparation results in alteration of chromosome morphology and chromosome loss (f 2n = 25, g 2n = 29). h Chromosome spreads obtained using 128-cell embryos imaged under lower magnifications. All blastomeres were synchronously arrested at metaphase. i Quantification of the number of chromosomes per cell