From: Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography
(a) | |||||||
τD (ms) | E0 (mmol/L) | S0 (mmol/L) | k1 (L mmol−1 s−1) | k−1 (s−1) | k2 (s−1) | k3 (s−1) | IP (mmol/L) |
15/1.5 | 25 | 200 | 3.2 | 0.01 | 7 | 0.8 | 6 |
(b) | |||||||
0 ms | 30 ms* | 100 ms | 500 ms | 2 s | |||
E (%) | 100 | 10/2 | 2 | 1 | 1 | ||
ES (%) | 0 | 84 | 58 | 19 | 59 | ||
E-CFO* (%) | 0 | 6/14 | 40 | 80 | 40 | ||
P ([mmol/L) | 0 | 0.05 | 0.5 | 5.8 | 23.4 | ||
(c) | |||||||
Shard-shaped crystals | Needle-shaped crystals | ||||||
BlaC concentration in crystal | 16 mmol/L | 30 mmol/L | |||||
Average size([μm3) | 10 × 10 × 3 | 5 × 2 × 2 | |||||
τD | 3.5 ms | 0.8 ms | |||||
Mixing ratio crystal:CEF | 1:4 | 1:4 | |||||
CEF = 200 mmol/L | |||||||
CEF after mixing | 160 mmol/L | 160 mmol/L | |||||
Time to reach stoichiometric conc. | 0.36 ms | 0.17 ms | |||||
CEF = 300 mmol/L | |||||||
CEF after mixing | 240 mmol/L | 240 mmol/L | |||||
Time to reach stoichiometric conc. | 0.24 ms | 0.10 ms |