Fig. 5

Neuronal survival is compromised in YAC128 1:3 CS co-cultures. DIV21 WT and YAC128 co-cultures were fixed at DIV21 and stained for MAP2 and DARPP32 (D32). (a) Sample fields of view at 20X objective (scale bar = 100 μm). The numbers of (Bi) MAP2+ and (Bii) DARPP32+ neurons per field of view are reduced in YAC128 1:3 co-cultures. (Biii) The proportion of DARPP32+ neurons (# DARPP32+ divided by # MAP2+) surviving at DIV21 is also significantly lower in YAC128 1:3 co-cultures [n = 30 fields of view from three independent cultures; two-way ANOVA with Bonferroni post-hoc analysis; *p < 0.05, ***p < 0.001]