Fig. 4
The extended acid patch of H2A.Z is important for the proper H3K27me3 level in mES cells. a Rescuing the global level of H3K27me3 in H2A.2 knockdown mES cells by various histones and its mutants (H2A, H2A.Z, H2A.Z D98N/S99K, and H2A.Z S99 K). b The dynamic changes of levels of H3K27me3 and H2A.Z in specific gene loci (Adra2c) upon knockdown of H2A.Z in mES cells. c Schematic view of the Adra2c promoter region. Arrows indicate the positions for primers. d Rescuing the deposition of H3K27me3 and the local chromatin compaction at the promoter of Adra2c in H2A.Z knockdown mES cells by various histones and its mutants (H2A, H2A.Z, H2A.Z D98N/S99K, and H2A.Z S99K). Levels of H3K27me3, H2A.Z, exogenous Flag-H2A.Z, and H3 at the promoter of Adra2c were monitored by ChIP-qPCR. The local chromatin compaction/dynamics at the promoter of Adra2c was analyzed by DNase protection assay via EpiQ™ kit. The p values were calculated with Student’s t test (*** < 0.01; * < 0.05; n = 3)