Fig. 4

Chryseobacterium nematophagum degrade the collagenous matrix of Caenorhabditis elegans. COL-12 TY tagged C. elegans strains (IA132) were incubated with C. nematophagum JUb129 for 24 h (1) 48 h (3) and with OP50 alone control for 48 h (2). IA132 was incubated for 48 h with non-pathogenic Chryseobacterium indologenes (4). IA132 adults were pre-cleared of OP50-1 by washing in M9 buffer and culturing on non-seeded plates for 4 h and then incubated for 48 h with C. nematophagum JUb129 (5) or JUb275 (6). Twenty adult worms per treatment were extracted and Western blotted and probed with anti-TY tag (upper) and then re-probed anti-β-actin (lower) antibodies