Fig. 5

Bundling time and contour length. a Average bundling times in theory (black line) and experiments (circles with error bars) measured as spindle reassembly time for d_SPB < 2.5 μm (n = 109, strain KI061; n = 42, strain LW042). The average bundling time, 〈t_B〉 = t_tot/n_B, where t_tot is the total time the MTs were observed or simulated and n_B is the number of bundling events in that time. b Average bundling times in experiments, for 1.5 μm < d_SPB < 2.5 μm (white bar, data from a), and in theory for varied parameters (colored bars, d_SPB = 2 μm). c Contour length difference, calculated as L₁ + L₂ − d_SPB, (main graph) and the number of motors (inset) as a function of time. In the simulations, MTs start from a symmetric configuration and α = 120°, y_min = 0 μm (see Methods). Average value (black line), standard deviation (shaded area), 5 sample paths for simulations (colored lines) for d_SPB = 2 μm, R_1,2 = 2 μm, and n_MT = 2. Mean experimental values (white dots) from Fig. 2d (from cells where the motors were not tagged in order to avoid potential effects of tagging on motor velocity), with times shifted by 16 s. d Contour velocity in experiments (white bar; data from Fig. 2d) and in theory for varied parameters (colored bars); R_1,2 = 2 μm, d_SPB = 2 μm, except for the last bar R_1,2 = 1 μm, d_SPB = 1 μm. e Contour length difference (main graph) and the number of passive crosslinkers (inset) as a function of time, with same color code as in c. Parameters for passive crosslinkers: diffusion constant D_c = 0.05 μm²/s [61], and the rest length y₀ = 40 nm [62]. f Average contour length difference from theory for passive crosslinkers (red) and motors (blue) as a function of time, for parameters given in the legend and in e. In panels d–f, initial geometry is the same as in c. Parameters in all panels are from Table 1 unless stated otherwise. Error bars, s.e.m