Fig. 8

Subcellular function of MOV10 in germ cells. Diagram of proposed RNA regulatory mechanisms executed by MOV10 in interaction with diverse RNA species and multiple proteins. MOV10-mediated RNA regulation pathways are initiated in the nucleus, where MOV10 associates with splicing factors, such as SRSF1 and DDX5, to bind intronic regions of pre-mRNA. The splicing process is mechanistically coupled with transcription and RNA processing, linking directly or indirectly to the fate of nascent transcripts for miRNA, lncRNA, mRNA, or even 3′-UTR. In the case of Mov10 deficiency, this coordinated regulation is severely impaired, globally impairing RNA splicing/processing activity, and thus failure to maintain a normal balance of various RNA pools, for example, as illustrated here, MOV10-mediated splicing regulation of intronic miRNAs. MOV10 transports its target RNAs from the nucleus to the cytoplasm to carry out further maturation, degradation, or translation. MOV10 predominantly binds long 3′-UTR as a mediator of mRNA decay via interaction with the NMD factor UPF1. Thus, the disruption of MOV10-mediated RNA homeostasis could reflect a profound, combinatorial effect resulting from a cascade of hierarchical events causing RNA dysregulation